Genotyping and primer details


Genotyping and primer details for 266B08 - At4g14710
Line ID 266B08
Locus At4g14710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8425344 of line 266B08 Chr4:8425344
   
Insertion allele amplicon 8474 - 27sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 27sm: 5'-GTCTTTTCTTGGATGAACTTTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8425004
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11