Genotyping and primer details


Genotyping and primer details for 266D04 - At5g45600
Line ID 266D04
Locus At5g45600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18489655 of line 266D04 Chr5:18489655
   
Insertion allele amplicon 8474 - AC15
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AC15: 5'-TGTTTTGGGTGGATACAACAGATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18489049
   
wt amplicon (over InsPos) 03vu - AC15
wt amplicon start Chr5:18489049
wt amplicon end Chr5:18490482
wt amplicon size 1434 bp
Locus specific primer AC15: 5'-TGTTTTGGGTGGATACAACAGATA-3'
2nd locus specific primer 03vu: 5'-TTTTGGACAGAAGACACAAAGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11