Genotyping and primer details


Genotyping and primer details for 269C11 - At4g09150
Line ID 269C11
Locus At4g09150 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5832150 of line 269C11 Chr4:5832150
   
Insertion allele amplicon 8474 - 07wu
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07wu: 5'-CAGGTGAATTTTACCAGTTGGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5832603
   
wt amplicon (over InsPos) 43xh - 07wu
wt amplicon start Chr4:5831433
wt amplicon end Chr4:5832603
wt amplicon size 1171 bp
Locus specific primer 07wu: 5'-CAGGTGAATTTTACCAGTTGGTTT-3'
2nd locus specific primer 43xh: 5'-AGTCAGGGATGAACTCTGTGAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11