Genotyping and primer details


Genotyping and primer details for 270D06 - At3g11850
Line ID 270D06
Locus At3g11850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:3739599 of line 270D06 Chr3:3739599
   
Insertion allele amplicon 8474 - 18gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18gp: 5'-AATACATTTTCTCTGCAACCCAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:3739063
   
wt amplicon (over InsPos) 28hs - 18gp
wt amplicon start Chr3:3739063
wt amplicon end Chr3:3740119
wt amplicon size 1057 bp
Locus specific primer 18gp: 5'-AATACATTTTCTCTGCAACCCAAG-3'
2nd locus specific primer 28hs: 5'-ATGACTTGTCGGAAATAGGAGATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11