Genotyping and primer details


Genotyping and primer details for 271A03 - At1g19500
Line ID 271A03
Locus At1g19500 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6754973 of line 271A03 Chr1:6754973
   
Insertion allele amplicon 8474 - KR46
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KR46: 5'-GTTCGGTGATAAAGCCAAGTATGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6755333
   
wt amplicon (over InsPos) 19sq - KR46
wt amplicon start Chr1:6754681
wt amplicon end Chr1:6755333
wt amplicon size 653 bp
Locus specific primer KR46: 5'-GTTCGGTGATAAAGCCAAGTATGT-3'
2nd locus specific primer 19sq: 5'-GTTAACGGATATAGAAATGGGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11