Genotyping and primer details


Genotyping and primer details for 271A12 - At1g21920
Line ID 271A12
Locus At1g21920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7705730 of line 271A12 Chr1:7705730
   
Insertion allele amplicon 8474 - 32by
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 32by: 5'-CTAGCCAATCTCCTTCATAACGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7705245
   
wt amplicon (over InsPos) 06dg - 32by
wt amplicon start Chr1:7705245
wt amplicon end Chr1:7706073
wt amplicon size 829 bp
Locus specific primer 32by: 5'-CTAGCCAATCTCCTTCATAACGTC-3'
2nd locus specific primer 06dg: 5'-ATTAACGAAGAAAGCGAAAAGTCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11