Genotyping and primer details


Genotyping and primer details for 274C02 - At2g35020
Line ID 274C02
Locus At2g35020 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:14757753 of line 274C02 Chr2:14757753
   
Insertion allele amplicon 8474 - 78cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 78cs: 5'-ACTTCCGTTCATTGAGTTCTGTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:14757278
   
wt amplicon (over InsPos) 03dn - 78cs
wt amplicon start Chr2:14757278
wt amplicon end Chr2:14758215
wt amplicon size 938 bp
Locus specific primer 78cs: 5'-ACTTCCGTTCATTGAGTTCTGTTT-3'
2nd locus specific primer 03dn: 5'-CAGCAAAGACAGAACTGAAATGAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11