Genotyping and primer details


Genotyping and primer details for 275F04 - At1g69740
Line ID 275F04
Locus At1g69740 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26234697 of line 275F04 Chr1:26234697
   
Insertion allele amplicon 8474 - 19sj
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 19sj: 5'-TCAATGGAATCTACAAGAACCACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26234927
   
wt amplicon (over InsPos) 23tc - 19sj
wt amplicon start Chr1:26234443
wt amplicon end Chr1:26234927
wt amplicon size 485 bp
Locus specific primer 19sj: 5'-TCAATGGAATCTACAAGAACCACA-3'
2nd locus specific primer 23tc: 5'-ATTGCTGCATACCAAGTACTTTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11