Genotyping and primer details

Genotyping and primer details for 276A01 - At3g02530
Line ID 276A01
Locus At3g02530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:529945 of line 276A01 Chr3:529945
Insertion allele amplicon 8474 - 19gp
Locus specific primer 19gp: 5'-TTTGATCTTTGTGGAGAAGAAGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:529530
wt amplicon (over InsPos) 26hs - 19gp
wt amplicon start Chr3:529530
wt amplicon end Chr3:530455
wt amplicon size 926 bp
Locus specific primer 19gp: 5'-TTTGATCTTTGTGGAGAAGAAGGT-3'
2nd locus specific primer 26hs: 5'-ATTGAGCTGAAGAACAAGGTGAGT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11