Genotyping and primer details


Genotyping and primer details for 283D10 - At1g78490
Line ID 283D10
Locus At1g78490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:29530144 of line 283D10 Chr1:29530144
   
Insertion allele amplicon 8474 - 18ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18ah: 5'-ACAGCCGCAAAGAGTACATAGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29530611
   
Insertion allele amplicon 8474 - CQ29
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ29: 5'-AACATGACTTTCACCAACATGGTA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:29529700
   
wt amplicon (over InsPos) 18ah - CQ29
wt amplicon start Chr1:29529700
wt amplicon end Chr1:29530611
wt amplicon size 912 bp
Locus specific primer CQ29: 5'-AACATGACTTTCACCAACATGGTA-3'
2nd locus specific primer 18ah: 5'-ACAGCCGCAAAGAGTACATAGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11