Genotyping and primer details


Genotyping and primer details for 287A07 - At1g11100
Line ID 287A07
Locus At1g11100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3704998 of line 287A07 Chr1:3704998
   
Insertion allele amplicon 8474 - 42ac
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42ac: 5'-CCAAGGAAGAACGTGATTTCTACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3705550
   
wt amplicon (over InsPos) 03si - 42ac
wt amplicon start Chr1:3704662
wt amplicon end Chr1:3705550
wt amplicon size 889 bp
Locus specific primer 42ac: 5'-CCAAGGAAGAACGTGATTTCTACT-3'
2nd locus specific primer 03si: 5'-TGTCAAGCTGCTGATTATTCTCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11