Genotyping and primer details


Genotyping and primer details for 288A08 - At1g59660
Line ID 288A08
Locus At1g59660 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:21928628 of line 288A08 Chr1:21928628
   
Insertion allele amplicon 8474 - 20ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 20ah: 5'-AGTCTACACACATCCGTCTCTCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21928920
   
Insertion allele amplicon 8474 - CQ34
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer CQ34: 5'-TTTTCTGAATTTTGGCTTTGTCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:21928057
   
wt amplicon (over InsPos) 20ah - CQ34
wt amplicon start Chr1:21928057
wt amplicon end Chr1:21928920
wt amplicon size 864 bp
Locus specific primer CQ34: 5'-TTTTCTGAATTTTGGCTTTGTCTT-3'
2nd locus specific primer 20ah: 5'-AGTCTACACACATCCGTCTCTCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11