Genotyping and primer details


Genotyping and primer details for 291F05 - At3g20540
Line ID 291F05
Locus At3g20540 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:7172078 of line 291F05 Chr3:7172078
   
Insertion allele amplicon 8474 - PF70
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer PF70: 5'-AGTTAGCTTGAATGGAGATGCAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:7171768
   
wt amplicon (over InsPos) 39dy - PF70
wt amplicon start Chr3:7171768
wt amplicon end Chr3:7172573
wt amplicon size 806 bp
Locus specific primer PF70: 5'-AGTTAGCTTGAATGGAGATGCAAT-3'
2nd locus specific primer 39dy: 5'-AAGCAAAGGCATCCTGAATAAGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11