Genotyping and primer details


Genotyping and primer details for 291F08 - At4g08930
Line ID 291F08
Locus At4g08930 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:5730129 of line 291F08 Chr4:5730129
   
Insertion allele amplicon 8474 - 10nt
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 10nt: 5'-TCACTGTGAAGCTGAAACACTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:5730724
   
wt amplicon (over InsPos) 37oj - 10nt
wt amplicon start Chr4:5729805
wt amplicon end Chr4:5730724
wt amplicon size 920 bp
Locus specific primer 10nt: 5'-TCACTGTGAAGCTGAAACACTTCT-3'
2nd locus specific primer 37oj: 5'-AGAATAAGTGTGACTATGCGGCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11