Genotyping and primer details


Genotyping and primer details for 293G01 - At3g13570
Line ID 293G01
Locus At3g13570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4430427 of line 293G01 Chr3:4430427
   
Insertion allele amplicon 8474 - 21hw
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21hw: 5'-GGGTGAGGATGCTCTGTAGATAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4431046
   
wt amplicon (over InsPos) 17ix - 21hw
wt amplicon start Chr3:4429891
wt amplicon end Chr3:4431046
wt amplicon size 1156 bp
Locus specific primer 21hw: 5'-GGGTGAGGATGCTCTGTAGATAAG-3'
2nd locus specific primer 17ix: 5'-TCTGTCAGTATGAATTTCACAGGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11