Genotyping and primer details


Genotyping and primer details for 294E02 - At5g65330
Line ID 294E02
Locus At5g65330 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26111090 of line 294E02 Chr5:26111090
   
Insertion allele amplicon 8474 - 08ou
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 08ou: 5'-TATTAGTTGATTCAGACAAGAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26111727
   
wt amplicon (over InsPos) 12vu - 08ou
wt amplicon start Chr5:26110346
wt amplicon end Chr5:26111727
wt amplicon size 1382 bp
Locus specific primer 08ou: 5'-TATTAGTTGATTCAGACAAGAAG-3'
2nd locus specific primer 12vu: 5'-TTCTTCGTCACGTAATTCAACAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11