Genotyping and primer details


Genotyping and primer details for 294E10 - At4g14910
Line ID 294E10
Locus At4g14910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8528549 of line 294E10 Chr4:8528549
   
Insertion allele amplicon 8474 - 07jr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 07jr: 5'-AGGAACATACGACACTCAGGTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8529045
   
wt amplicon (over InsPos) 20kj - 07jr
wt amplicon start Chr4:8528232
wt amplicon end Chr4:8529045
wt amplicon size 814 bp
Locus specific primer 07jr: 5'-AGGAACATACGACACTCAGGTTCT-3'
2nd locus specific primer 20kj: 5'-CATGACCAAAACAAAATGATTACG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11