Genotyping and primer details


Genotyping and primer details for 294E12 - At2g03640
Line ID 294E12
Locus At2g03640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:1105517 of line 294E12 Chr2:1105517
   
Insertion allele amplicon 8474 - 31sm
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 31sm: 5'-CAGATTTTTAAGCGTGTGATTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:1105046
   
wt amplicon (over InsPos) 16ti - 31sm
wt amplicon start Chr2:1105046
wt amplicon end Chr2:1105793
wt amplicon size 748 bp
Locus specific primer 31sm: 5'-CAGATTTTTAAGCGTGTGATTGAA-3'
2nd locus specific primer 16ti: 5'-TAATGGTTTTGATTAGCAGAGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11