Genotyping and primer details


Genotyping and primer details for 294G07 - At2g28320
Line ID 294G07
Locus At2g28320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:12098597 of line 294G07 Chr2:12098597
   
Insertion allele amplicon 8474 - 45fr
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 45fr: 5'-TTCTTTCCCACACTTTGCTTAACT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:12098929
   
wt amplicon (over InsPos) 25go - 45fr
wt amplicon start Chr2:12098153
wt amplicon end Chr2:12098929
wt amplicon size 777 bp
Locus specific primer 45fr: 5'-TTCTTTCCCACACTTTGCTTAACT-3'
2nd locus specific primer 25go: 5'-ACTGATCCTAGTTGTGATTTGCCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11