Genotyping and primer details


Genotyping and primer details for 295A02 - At3g24506
Line ID 295A02
Locus At3g24506 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8929307 of line 295A02 Chr3:8929307
   
Insertion allele amplicon 8474 - 05cz
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05cz: 5'-GTATGATCTACACAGGCACAGGAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8928827
   
wt amplicon (over InsPos) 19dk - 05cz
wt amplicon start Chr3:8928827
wt amplicon end Chr3:8929719
wt amplicon size 893 bp
Locus specific primer 05cz: 5'-GTATGATCTACACAGGCACAGGAC-3'
2nd locus specific primer 19dk: 5'-AAAAATGCAAACAAACACTTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11