Genotyping and primer details


Genotyping and primer details for 296F07 - At2g41490
Line ID 296F07
Locus At2g41490 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:17301876 of line 296F07 Chr2:17301876
   
Insertion allele amplicon 8474 - SR42
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer SR42: 5'-AATATTATCGCCACGATCAAAAAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:17302279
   
wt amplicon (over InsPos) 03bv - SR42
wt amplicon start Chr2:17301452
wt amplicon end Chr2:17302279
wt amplicon size 828 bp
Locus specific primer SR42: 5'-AATATTATCGCCACGATCAAAAAG-3'
2nd locus specific primer 03bv: 5'-TTATGTCATGCTGATGTGTTGCTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11