Genotyping and primer details


Genotyping and primer details for 297C03 - At4g26990
Line ID 297C03
Locus At4g26990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:13551195 of line 297C03 Chr4:13551195
   
Insertion allele amplicon 8474 - FV82
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer FV82: 5'-TTCTAATCGAGGAATATTAGCGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:13550679
   
wt amplicon (over InsPos) 19si - FV82
wt amplicon start Chr4:13550679
wt amplicon end Chr4:13551759
wt amplicon size 1081 bp
Locus specific primer FV82: 5'-TTCTAATCGAGGAATATTAGCGGA-3'
2nd locus specific primer 19si: 5'-TCTTTTTACTGTCCTCCAGGTGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11