Genotyping and primer details


Genotyping and primer details for 302E01 - At4g02850
Line ID 302E01
Locus At4g02850 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:1267880 of line 302E01 Chr4:1267880
   
Insertion allele amplicon 8474 - 03bn
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 03bn: 5'-GAAAATTCGACAGTGTCAGATGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:1267414
   
wt amplicon (over InsPos) 09cr - 03bn
wt amplicon start Chr4:1267414
wt amplicon end Chr4:1268170
wt amplicon size 757 bp
Locus specific primer 03bn: 5'-GAAAATTCGACAGTGTCAGATGAA-3'
2nd locus specific primer 09cr: 5'-GCTTCCACTTGATTAGATCTGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11