Genotyping and primer details


Genotyping and primer details for 305H10 - At3g58640
Line ID 305H10
Locus At3g58640 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21687583 of line 305H10 Chr3:21687583
   
Insertion allele amplicon 8474 - 05ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05ie: 5'-CTTCCATACAAAACCGTGTATCCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21687955
   
wt amplicon (over InsPos) 06jm - 05ie
wt amplicon start Chr3:21687044
wt amplicon end Chr3:21687955
wt amplicon size 912 bp
Locus specific primer 05ie: 5'-CTTCCATACAAAACCGTGTATCCT-3'
2nd locus specific primer 06jm: 5'-ACTCAAAGGAGAAAGCCAACAATA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11