Genotyping and primer details


Genotyping and primer details for 307A12 - At5g05620
Line ID 307A12
Locus At5g05620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:1679939 of line 307A12 Chr5:1679939
   
Insertion allele amplicon 8474 - 17vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17vf: 5'-AGCGGATGACCAACACTATATACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:1679619
   
wt amplicon (over InsPos) 20vu - 17vf
wt amplicon start Chr5:1679619
wt amplicon end Chr5:1680531
wt amplicon size 913 bp
Locus specific primer 17vf: 5'-AGCGGATGACCAACACTATATACC-3'
2nd locus specific primer 20vu: 5'-CTGCCAAGAACAATTCACATAAGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11