Genotyping and primer details


Genotyping and primer details for 307C10 - At1g04810
Line ID 307C10
Locus At1g04810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:1350360 of line 307C10 Chr1:1350360
   
Insertion allele amplicon 8474 - 16et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 16et: 5'-TGATGCTGATCAAACTCTTCATCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:1350871
   
wt amplicon (over InsPos) 06fo - 16et
wt amplicon start Chr1:1350019
wt amplicon end Chr1:1350871
wt amplicon size 853 bp
Locus specific primer 16et: 5'-TGATGCTGATCAAACTCTTCATCT-3'
2nd locus specific primer 06fo: 5'-TAGACCCAATTTTGTATTCCGACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11