Genotyping and primer details


Genotyping and primer details for 307F10 - At1g33260
Line ID 307F10
Locus At1g33260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12065086 of line 307F10 Chr1:12065086
   
Insertion allele amplicon 8474 - 80cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 80cs: 5'-TCGTAGTGGTTATAACGTCAGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12064475
   
wt amplicon (over InsPos) 05dw - 80cs
wt amplicon start Chr1:12064475
wt amplicon end Chr1:12065558
wt amplicon size 1084 bp
Locus specific primer 80cs: 5'-TCGTAGTGGTTATAACGTCAGTGC-3'
2nd locus specific primer 05dw: 5'-TGCACAATCTGAGGACTACATTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11