Genotyping and primer details


Genotyping and primer details for 312H02 - At1g52260
Line ID 312H02
Locus At1g52260 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19461364 of line 312H02 Chr1:19461364
   
Insertion allele amplicon 8474 - 21gp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21gp: 5'-TTTCACAATCATCATCGCTTACTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19460762
   
wt amplicon (over InsPos) 22gp - 21gp
wt amplicon start Chr1:19460762
wt amplicon end Chr1:19461983
wt amplicon size 1221 bp
Locus specific primer 21gp: 5'-TTTCACAATCATCATCGCTTACTC-3'
2nd locus specific primer 22gp: 5'-AGCATAACCTGACAAAGGAAGAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11