Genotyping and primer details


Genotyping and primer details for 313G10 - At2g06990
Line ID 313G10
Locus At2g06990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:2897217 of line 313G10 Chr2:2897217
   
Insertion allele amplicon 8474 - AH26
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AH26: 5'-CAGAAACCGTAAATGTTTTGGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:2896586
   
Insertion allele amplicon 8474 - AP23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer AP23: 5'-AATGTATACTTACCAGCCGACCAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:2897736
   
wt amplicon (over InsPos) AP23 - AH26
wt amplicon start Chr2:2896586
wt amplicon end Chr2:2897736
wt amplicon size 1151 bp
Locus specific primer AH26: 5'-CAGAAACCGTAAATGTTTTGGAAT-3'
2nd locus specific primer AP23: 5'-AATGTATACTTACCAGCCGACCAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11