Genotyping and primer details


Genotyping and primer details for 316D12 - At1g18620
Line ID 316D12
Locus At1g18620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:6410844 of line 316D12 Chr1:6410844
   
Insertion allele amplicon 8474 - 15dl
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15dl: 5'-GATGAACACGATGATGAGAAAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:6411437
   
wt amplicon (over InsPos) 08hs - 15dl
wt amplicon start Chr1:6410457
wt amplicon end Chr1:6411437
wt amplicon size 981 bp
Locus specific primer 15dl: 5'-GATGAACACGATGATGAGAAAGAA-3'
2nd locus specific primer 08hs: 5'-ATATGAAATATGGAAAGTGGCGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11