Genotyping and primer details


Genotyping and primer details for 316H09 - At3g13830
Line ID 316H09
Locus At3g13830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:4554739 of line 316H09 Chr3:4554739
   
Insertion allele amplicon 8474 - 52sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 52sf: 5'-TGCTTGTTGGAGGGATTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:4554354
   
wt amplicon (over InsPos) 01tc - 52sf
wt amplicon start Chr3:4554354
wt amplicon end Chr3:4555183
wt amplicon size 830 bp
Locus specific primer 52sf: 5'-TGCTTGTTGGAGGGATTC-3'
2nd locus specific primer 01tc: 5'-CATGAAAAAGCAGAATTGTTCAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11