Genotyping and primer details


Genotyping and primer details for 317B08 - At2g22910
Line ID 317B08
Locus At2g22910 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9750712 of line 317B08 Chr2:9750712
   
Insertion allele amplicon 8474 - BV22
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV22: 5'-GTACCTTCGTCGTTACTATCTCCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9750268
   
wt amplicon (over InsPos) 22sl - BV22
wt amplicon start Chr2:9750268
wt amplicon end Chr2:9751051
wt amplicon size 784 bp
Locus specific primer BV22: 5'-GTACCTTCGTCGTTACTATCTCCG-3'
2nd locus specific primer 22sl: 5'-GCAAAGAAGTTACCAGTATCGACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11