Genotyping and primer details


Genotyping and primer details for 319H09 - At1g34110
Line ID 319H09
Locus At1g34110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12418562 of line 319H09 Chr1:12418562
   
Insertion allele amplicon 8474 - EQ18
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ18: 5'-CATCGTAGCAGTGAAGAAACTCTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12418862
   
Insertion allele amplicon 8474 - KH53
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KH53: 5'-AAAACAATCTTTTGCCAATAATGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12418125
   
wt amplicon (over InsPos) KH53 - EQ18
wt amplicon start Chr1:12418125
wt amplicon end Chr1:12418862
wt amplicon size 738 bp
Locus specific primer EQ18: 5'-CATCGTAGCAGTGAAGAAACTCTG-3'
2nd locus specific primer KH53: 5'-AAAACAATCTTTTGCCAATAATGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11