Genotyping and primer details


Genotyping and primer details for 325B05 - At5g62520
Line ID 325B05
Locus At5g62520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25098832 of line 325B05 Chr5:25098832
   
Insertion allele amplicon 8474 - JK36
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer JK36: 5'-TGGTGATCAATGTCAGATTCTCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25098259
   
wt amplicon (over InsPos) 09sq - JK36
wt amplicon start Chr5:25098259
wt amplicon end Chr5:25099177
wt amplicon size 919 bp
Locus specific primer JK36: 5'-TGGTGATCAATGTCAGATTCTCTC-3'
2nd locus specific primer 09sq: 5'-GCTCCGTGTAATTCTCCTATTCTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11