Genotyping and primer details


Genotyping and primer details for 325C11 - At2g01210
Line ID 325C11
Locus At2g01210 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:120317 of line 325C11 Chr2:120317
   
Insertion allele amplicon 8474 - EQ23
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer EQ23: 5'-GATCCCATAAGAGTAAACATCCCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:119782
   
Insertion allele amplicon 8474 - KH56
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer KH56: 5'-GAAACCAAACAGAAATCAAGTGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:120813
   
wt amplicon (over InsPos) KH56 - EQ23
wt amplicon start Chr2:119782
wt amplicon end Chr2:120813
wt amplicon size 1032 bp
Locus specific primer EQ23: 5'-GATCCCATAAGAGTAAACATCCCA-3'
2nd locus specific primer KH56: 5'-GAAACCAAACAGAAATCAAGTGGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11