Genotyping and primer details


Genotyping and primer details for 326G10 - At2g26770
Line ID 326G10
Locus At2g26770 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:11406073 of line 326G10 Chr2:11406073
   
Insertion allele amplicon 8474 - 05md
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 05md: 5'-CATGCAGTTTTTGTTAGTCCTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:11406566
   
wt amplicon (over InsPos) 11mx - 05md
wt amplicon start Chr2:11405577
wt amplicon end Chr2:11406566
wt amplicon size 990 bp
Locus specific primer 05md: 5'-CATGCAGTTTTTGTTAGTCCTTGA-3'
2nd locus specific primer 11mx: 5'-GGGATATATTTTCCTCGCTCTTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11