Genotyping and primer details


Genotyping and primer details for 326H06 - At2g24800
Line ID 326H06
Locus At2g24800 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10571429 of line 326H06 Chr2:10571429
   
Insertion allele amplicon 8474 - GD84
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GD84: 5'-CCGATTTCACAGTTGATAAGATGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10571831
   
Insertion allele amplicon 8474 - GD85
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GD85: 5'-GATACATTAACGTTTGACGGGAGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10570899
   
wt amplicon (over InsPos) 40rd - GD85
wt amplicon start Chr2:10570899
wt amplicon end Chr2:10571698
wt amplicon size 800 bp
Locus specific primer GD85: 5'-GATACATTAACGTTTGACGGGAGT-3'
2nd locus specific primer 40rd: 5'-TCAATTTTCGATCTATTACGGGTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11