Genotyping and primer details


Genotyping and primer details for 328D02 - At5g64960
Line ID 328D02
Locus At5g64960 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25957062 of line 328D02 Chr5:25957062
   
Insertion allele amplicon 8474 - 11hg
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 11hg: 5'-CATTTTCTCAAGTAGTTCAAGGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25957594
   
wt amplicon (over InsPos) 15ig - 11hg
wt amplicon start Chr5:25956674
wt amplicon end Chr5:25957594
wt amplicon size 921 bp
Locus specific primer 11hg: 5'-CATTTTCTCAAGTAGTTCAAGGGC-3'
2nd locus specific primer 15ig: 5'-AAAAACCCTTATTGTTCCGACTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11