Genotyping and primer details


Genotyping and primer details for 335B03 - At5g64190
Line ID 335B03
Locus At5g64190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25680515 of line 335B03 Chr5:25680515
   
Insertion allele amplicon 8474 - 23vf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23vf: 5'-ACTCCAATCTACGAGTCTCATGGT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25681121
   
wt amplicon (over InsPos) 43vu - 23vf
wt amplicon start Chr5:25679871
wt amplicon end Chr5:25681121
wt amplicon size 1251 bp
Locus specific primer 23vf: 5'-ACTCCAATCTACGAGTCTCATGGT-3'
2nd locus specific primer 43vu: 5'-TCTACGAAGCTCCTCTGTATCTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11