Genotyping and primer details


Genotyping and primer details for 340B08 - At1g70985
Line ID 340B08
Locus At1g70985 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:26764986 of line 340B08 Chr1:26764986
   
Insertion allele amplicon 8474 - 12ah
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 12ah: 5'-ATGAATAAACGTTACGGTCACCTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26765181
   
Insertion allele amplicon 8474 - 42wp
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 42wp: 5'-TTTTTAATTATATCAAGCGGTGGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26765509
   
Insertion allele amplicon 8474 - BV48
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV48: 5'-GAACGTATGATTCAGTTCGCTACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:26764308
   
wt amplicon (over InsPos) 12ah - BV48
wt amplicon start Chr1:26764308
wt amplicon end Chr1:26765181
wt amplicon size 874 bp
Locus specific primer BV48: 5'-GAACGTATGATTCAGTTCGCTACA-3'
2nd locus specific primer 12ah: 5'-ATGAATAAACGTTACGGTCACCTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11