Genotyping and primer details


Genotyping and primer details for 341G12 - At4g38570
Line ID 341G12
Locus At4g38570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:18033147 of line 341G12 Chr4:18033147
   
Insertion allele amplicon 8474 - 15sf
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 15sf: 5'-TGATGTTGGTACCACAAGGATAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:18032827
   
wt amplicon (over InsPos) 09su - 15sf
wt amplicon start Chr4:18032827
wt amplicon end Chr4:18033567
wt amplicon size 741 bp
Locus specific primer 15sf: 5'-TGATGTTGGTACCACAAGGATAAA-3'
2nd locus specific primer 09su: 5'-TATGCAAATTTTCAGTGTTGAAGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11