Genotyping and primer details


Genotyping and primer details for 342A06 - At1g30920
Line ID 342A06
Locus At1g30920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11005025 of line 342A06 Chr1:11005025
   
Insertion allele amplicon 8474 - GD63
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GD63: 5'-CCACACACTTAACTTTAGGGGAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11004587
   
wt amplicon (over InsPos) 39rd - GD63
wt amplicon start Chr1:11004587
wt amplicon end Chr1:11005501
wt amplicon size 915 bp
Locus specific primer GD63: 5'-CCACACACTTAACTTTAGGGGAAA-3'
2nd locus specific primer 39rd: 5'-TTTTCCATAATCCAGAGATTCACC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11