Genotyping and primer details


Genotyping and primer details for 343G06 - At3g63460
Line ID 343G06
Locus At3g63460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23434787 of line 343G06 Chr3:23434787
   
Insertion allele amplicon 8474 - BM87
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BM87: 5'-AAATTGGCATCTACAATATCGAGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23435311
   
wt amplicon (over InsPos) 27sq - BM87
wt amplicon start Chr3:23434478
wt amplicon end Chr3:23435311
wt amplicon size 834 bp
Locus specific primer BM87: 5'-AAATTGGCATCTACAATATCGAGG-3'
2nd locus specific primer 27sq: 5'-ACAAGGATGGCATACTCAGTCTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11