Genotyping and primer details


Genotyping and primer details for 344F12 - At3g58760
Line ID 344F12
Locus At3g58760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21731529 of line 344F12 Chr3:21731529
   
Insertion allele amplicon 8474 - 21kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 21kh: 5'-ATGGCTCCAGAGGTTTATAGGAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21731001
   
wt amplicon (over InsPos) 09ky - 21kh
wt amplicon start Chr3:21731001
wt amplicon end Chr3:21732040
wt amplicon size 1040 bp
Locus specific primer 21kh: 5'-ATGGCTCCAGAGGTTTATAGGAAT-3'
2nd locus specific primer 09ky: 5'-GCTCGAGGTCTTCCTATTCTTTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11