Genotyping and primer details


Genotyping and primer details for 345B09 - At5g66310
Line ID 345B09
Locus At5g66310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26489384 of line 345B09 Chr5:26489384
   
Insertion allele amplicon 8474 - 09ie
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 09ie: 5'-TTCAAAGATATGTACCACTGTCGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26489067
   
wt amplicon (over InsPos) 10ji - 09ie
wt amplicon start Chr5:26489067
wt amplicon end Chr5:26489802
wt amplicon size 736 bp
Locus specific primer 09ie: 5'-TTCAAAGATATGTACCACTGTCGC-3'
2nd locus specific primer 10ji: 5'-AAAGACGTACACTATGAGCGGAAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11