Genotyping and primer details


Genotyping and primer details for 346F04 - At2g37160
Line ID 346F04
Locus At2g37160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15611022 of line 346F04 Chr2:15611022
   
Insertion allele amplicon 8474 - 18et
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 18et: 5'-AAATGCAATAGGGAAGATCATTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15611339
   
wt amplicon (over InsPos) 14fo - 18et
wt amplicon start Chr2:15610565
wt amplicon end Chr2:15611339
wt amplicon size 775 bp
Locus specific primer 18et: 5'-AAATGCAATAGGGAAGATCATTTG-3'
2nd locus specific primer 14fo: 5'-TACTAGTATAGCTTGGGTGCCTGG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11