Genotyping and primer details


Genotyping and primer details for 350A02 - At4g00520
Line ID 350A02
Locus At4g00520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:231087 of line 350A02 Chr4:231087
   
Insertion allele amplicon 8474 - BV65
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer BV65: 5'-TTCTTTTGTCTTAGCGAAGTGTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:230614
   
wt amplicon (over InsPos) 30si - BV65
wt amplicon start Chr4:230614
wt amplicon end Chr4:231534
wt amplicon size 921 bp
Locus specific primer BV65: 5'-TTCTTTTGTCTTAGCGAAGTGTGA-3'
2nd locus specific primer 30si: 5'-ACAGATGCAGCAATTCTCTCACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11