Genotyping and primer details


Genotyping and primer details for 350E03 - At1g11170
Line ID 350E03
Locus At1g11170 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:3744007 of line 350E03 Chr1:3744007
   
Insertion allele amplicon 8474 - 17dh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 17dh: 5'-TTTGTGTGTTGTTGTTGTTCTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:3744456
   
wt amplicon (over InsPos) 12ee - 17dh
wt amplicon start Chr1:3743604
wt amplicon end Chr1:3744456
wt amplicon size 853 bp
Locus specific primer 17dh: 5'-TTTGTGTGTTGTTGTTGTTCTTGA-3'
2nd locus specific primer 12ee: 5'-ATGGATATGAAACTTGGGTACTGC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11