Genotyping and primer details


Genotyping and primer details for 351E08 - At4g15450
Line ID 351E08
Locus At4g15450 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:8840904 of line 351E08 Chr4:8840904
   
Insertion allele amplicon 8474 - GD50
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer GD50: 5'-CATGAGGGATAAATTTGGTAAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:8841289
   
wt amplicon (over InsPos) 38rd - GD50
wt amplicon start Chr4:8840608
wt amplicon end Chr4:8841289
wt amplicon size 682 bp
Locus specific primer GD50: 5'-CATGAGGGATAAATTTGGTAAGGA-3'
2nd locus specific primer 38rd: 5'-AATTGAGCAAGACAATGTTGAATG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11