Genotyping and primer details


Genotyping and primer details for 352E12 - At5g65620
Line ID 352E12
Locus At5g65620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:26224567 of line 352E12 Chr5:26224567
   
Insertion allele amplicon 8474 - 23kh
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 23kh: 5'-ATTCACCTGACGAAGACTAAAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:26224949
   
wt amplicon (over InsPos) 14ky - 23kh
wt amplicon start Chr5:26224115
wt amplicon end Chr5:26224949
wt amplicon size 835 bp
Locus specific primer 23kh: 5'-ATTCACCTGACGAAGACTAAAGGA-3'
2nd locus specific primer 14ky: 5'-GGAATTGATATTGAACCAGCAGAT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on Thursday, 06 December 2018 16:11